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dc.contributor.authorKhaskheli, Abid Ali
dc.contributor.authorTalpur, Farah Naz
dc.contributor.authorAydin, Aysun Cebeci
dc.contributor.authorJawaid, Sana
dc.contributor.authorSurhio, Muhammad Ali
dc.contributor.authorAfridi, Hassan Imran
dc.date.accessioned2020-02-14T09:13:21Z
dc.date.available2020-02-14T09:13:21Z
dc.date.issued2017en_US
dc.identifier.issn0916-8451
dc.identifier.othereISSN: 1347-6947
dc.identifier.otherDOI: 10.1080/09168451.2017.1356218
dc.identifier.urihttps://hdl.handle.net/20.500.12573/158
dc.descriptionAbid Ali Khaskheli greatly acknowledges the National Centre of Excellence in Analytical Chemistry, University of Sindh for providing monthly stipend to carry out this research work. No further funding was available.en_US
dc.description.abstractConjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate.en_US
dc.description.sponsorshipNational Centre of Excellence in Analytical Chemistry, University of Sindhen_US
dc.language.isoengen_US
dc.publisherTAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLANDen_US
dc.relation.ispartofseriesVolume: 81 Issue: 10 Pages: 2002-2008;
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcastor oilen_US
dc.subjectconjugated linoleic aciden_US
dc.subjectRhizopus oryzaeen_US
dc.subjectLactobacillus plantarumen_US
dc.titleOne-pot conjugated linoleic acid production from castor oil by Rhizopus oryzae lipase and resting cells of Lactobacillus plantarumen_US
dc.typearticleen_US
dc.contributor.departmentAGÜ, Mühendislik Fakültesi, Malzeme Bilimi ve Nanoteknoloji Mühendisliği Bölümüen_US
dc.contributor.institutionauthor
dc.identifier.doi10.1080/09168451.2017.1356218
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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