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dc.contributor.authorAllahverdiyev, Adil M.
dc.contributor.authorKoc, Rabia Cakir
dc.contributor.authorBagirova, Melahat
dc.contributor.authorElcicek, Serhat
dc.contributor.authorYesilkir Baydar, Serap
dc.contributor.authorOztel, Olga Nehir
dc.contributor.authorAbamor, Emrah Sefik
dc.contributor.authorAtes, Sezen Canim
dc.contributor.authorTopuzogullari, Murat
dc.contributor.authorDincer, Sevil Isoglu
dc.contributor.authorAkdeste, Zeynep
dc.date.accessioned2019-07-05T08:44:12Z
dc.date.available2019-07-05T08:44:12Z
dc.date.issued2017en_US
dc.identifier.citationASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE Volume: 10 Issue: 9 Pages: 877-886 DOI: 10.1016/j.apjtm.2017.09.001en_US
dc.identifier.issn1995-7645
dc.identifier.otherPubMed ID: 29080616
dc.identifier.otherAccession Number: WOS:000417215100006
dc.identifier.otherDOI: 10.1016/j.apjtm.2017.09.001
dc.identifier.urihttp://acikerisim.agu.edu.tr/xmlui/handle/20.500.12573/67
dc.descriptionIt is financially supported by TUBITAK (1085170SBAG-4007).en_US
dc.description.abstractObjective: To determine the antileishmanial vaccine effectiveness of lipophosphoglycan (LPG) and polyacrylic acids (PAA) conjugates on in vivo mice models. Methods: LPG molecule was isolated and purified from large-scale Leishmania donovani parasite culture. Protection efficacies of LPG alone, in combination with Freund's adjuvant, in a physical mixture and in conjugate (consisting of various LPG concentrations) with PAA, were comparatively determined by various techniques, such as cultivation with the micro-culture method, assessment of in vitro infection rates of peritoneal macrophages, determination of parasite load in liver with Leishman-Donovan Units, and detection of cytokine responses. Results: Obtained results demonstrated that the highest vaccine-mediated immune protection was provided by LPG-PAA conjugate due to all parameters investigated. According to the Leishman-Donovan Units results, the sharpest decline in parasite load was seen with a ratio of 81.17% when 35 mg LPG containing conjugate was applied. This value was 44.93% for the control group immunized only with LPG. Moreover, decreases in parasite load were 53.37%, 55.2% and 65.8% for the groups immunized with 10 mg LPG containing LPG-PAA conjugate, a physical mixture of the LPG-PAA, and a mixture of LPG + Freund's adjuvant, respectively. Furthermore, cytokine results supported that Th1 mediated protection occurred when mice were immunized with LPG-PAA conjugate. Conclusions: It has been demonstrated in this study that conjugate of LPG and PAA has an antileishmanial vaccine effect against visceral leishmaniasis. In this respect, the present study may lead to new vaccine approaches based on high immunogenic LPG molecule and adjuvant polymers in fighting against Leishmania infection.en_US
dc.description.sponsorshipTUBITAK - 1085170SBAG-4007en_US
dc.language.isoengen_US
dc.publisherELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDen_US
dc.relation.ispartofseriesASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE;Volume: 10 Issue: 9
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLeishmaniaen_US
dc.subjectLipophosphoglycanen_US
dc.subjectVaccineen_US
dc.subjectPolymeren_US
dc.subjectPolyacrylic aciden_US
dc.titleA new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugatesen_US
dc.typearticleen_US
dc.contributor.departmentAGÜ, Mühendislik Fakültesi, Malzeme Bilimi ve Nanoteknoloji Mühendisliği Bölümüen_US
dc.contributor.institutionauthor
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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