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dc.contributor.authorIcoz, Kutay
dc.contributor.authorSoylu, Mehmet Cagri
dc.contributor.authorCanikara, Zeynep
dc.contributor.authorUnal, Ekrem
dc.date.accessioned2021-05-18T07:50:04Z
dc.date.available2021-05-18T07:50:04Z
dc.date.issued2018en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.urihttps://doi.org/10.1002/elan.201700789
dc.identifier.urihttps://hdl.handle.net/20.500.12573/713
dc.descriptionAuthors acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK Project No: 115E020) for financial support and Prof. Servet Ozcan and Prof. Musa Karakukcu from Erciyes University for valuable discussions for the cell line related issues. Authors also acknowledge Tayyibe Gercek for growing cell cultures and Furkan Soysaldi for helping on preparation of QCM data.en_US
dc.description.abstractWe have investigated different surface functionalization methods to immobilize CD19 antibody on gold surface to capture B lymphoblast cells associated with the acute lymphoblastic leukemia disease. Quartz Crystal Microbalance measurements were performed to analyze the binding kinetics of each layer and determine the optimum method, which results in higher cell capture rates. The random orientation of antibody and oriented antibody through protein G was investigated and protein G presence resulted in 15,2Hz frequency shift for 10(4)cells/mL. The 3-mercaptopropyltrimethoxysilane (MPS) and 11-Mercaptoundecanoic acid (MUA) coatings of gold surface together with 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt (Sulfo-SMCC) and N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysulfosuccinimide (NHS) linker layers were tested on QCM for protein G and antibody binding. The results indicate that MUA, EDC/NHS, protein G, antibody CD19 is the optimum surface modification among the tested combinations. By using the optimum surface functionalization method, minimum 10(3) cell per mL was measured as 1.9Hz frequency shift.en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) 115E020en_US
dc.language.isoengen_US
dc.publisherWILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANYen_US
dc.relation.isversionof10.1002/elan.201700789en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectprotein Gen_US
dc.subjectB lymphoblasten_US
dc.subjectCD19 immobilizationen_US
dc.subjectquartz crystal microbalance with dissipationen_US
dc.subjectacute lymphoblastic leukemiaen_US
dc.subjectsurface functionalizationen_US
dc.titleQuartz-crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalizationen_US
dc.typearticleen_US
dc.contributor.departmentAGÜ, Mühendislik Fakültesi, Elektrik - Elektronik Mühendisliği Bölümüen_US
dc.contributor.authorID0000-0002-0947-6166en_US
dc.contributor.authorID0000-0001-5213-2679en_US
dc.contributor.authorID0000-0002-2691-4826en_US
dc.identifier.volumeVolume: 30 Issue: 5 Pages: 834-841en_US
dc.relation.journalELECTROANALYSISen_US
dc.relation.tubitak115E020
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US


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