Monitoring the Rhizopus oryzae lipase catalyzed hydrolysis of castor oil by ATR-FTIR spectroscopy

Abstract

A rapid and environmental friendly Attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopicmethod wasdevelopedformonitoring the Rhizopus oryzae lipase (ROL) catalyzedhydrolysis of castor oilinoil-in-water emulsionsystem.Acalibrationcurve was constructedusingpartialleast square (PLS) model by gravimetric addition of oleic acid (10–50%) in castor oil to detect the carbonyl absorption of free fatty acids (FFA) in the region (1690–1730 cm−1). The correlation co-efficient (R2) and root mean square error of calibration (RMSEC) by PLS model were found to be 0.999 and 0.316, respectively. ROL was found to be an efficient biocatalyst to produce free fatty acids (FFA) from castor oil. Factors affecting the rate of hydrolysis such as enzyme concentration (0.01%, w/v), pH (7), temperature (37 ◦C), oil–water ratio (1:4) and reaction time (12 h) were optimized. Under all set of conditions the ROL effectively hydrolyzed castor oil up to 90% yield of fatty acids. The methodology is fairly environmental friendly in both cases, i.e. using lipase for hydrolysis of castor oil and analyzing the product through FTIR spectroscopy