https://hdl.handle.net/20.500.12573/208 2026-05-08T07:20:37Z 2026-05-08T07:20:37Z Fidan, Ozkan Karipcin, Ayse Doga Kose, Ayse Hamide Anaz, Ayse Demirsoy, Beyza Nur Arslansoy, Nuriye Sun, Lei Mujwar, Somdutt https://hdl.handle.net/20.500.12573/2536 2025-06-17T07:24:42Z 2025-01-01T00:00:00Z

Discovery of a C-S lyase inhibitor for the prevention of human body malodor formation: tannic acid inhibits the thioalcohol production in Staphylococcus hominis Fidan, Ozkan; Karipcin, Ayse Doga; Kose, Ayse Hamide; Anaz, Ayse; Demirsoy, Beyza Nur; Arslansoy, Nuriye; Sun, Lei; Mujwar, Somdutt Human body odor is a result of the bacterial biotransformation of odorless precursor molecules secreted by the underarm sweat glands. In the human axilla, Staphylococcus hominis is the predominant bacterial species responsible for the biotransformation process of the odorless precursor molecule into the malodorous 3M3SH by two enzymes, a dipeptidase and a specific C-S lyase. The current solutions for malodor, such as deodorants and antiperspirants are known to block the apocrine glands or disrupt the skin microbiota. Additionally, these chemicals endanger both the environment and human health, and their long-term use can influence the function of sweat glands. Therefore, there is a need for the development of alternative, environmentally friendly, and natural solutions for the prevention of human body malodor. In this study, a library of secondary metabolites from various plants was screened to inhibit the C-S lyase, which metabolizes the odorless precursor sweat molecules, through molecular docking and molecular dynamics (MD) simulation. In silico studies revealed that tannic acid had the strongest affinity towards C-S lyase and was stably maintained in the binding pocket of the enzyme during 100-ns MD simulation. We found in the in vitro biotransformation assays that 1 mM tannic acid not only exhibited a significant reduction in malodor formation but also had quite low growth inhibition in S. hominis, indicating the minimum inhibitory effect of tannic acid on the skin microflora. This study paved the way for the development of a promising natural C-S lyase inhibitor to eliminate human body odor and can be used as a natural deodorizing molecule after further in vivo analysis.

2025-01-01T00:00:00Z Bozdag, Mehmet Urek, Ferhat Cesur, Sumeyye Sahin, Ali Gunduz, Oguzhan https://hdl.handle.net/20.500.12573/2514 2025-05-06T11:39:42Z 2025-01-01T00:00:00Z

Bovine serum albumin (BSA)-Loaded polyvinyl alcohol (PVA) / chitosan (CH) / hydroxyapatite (HA) electrospun nanofibers for bone tissue regeneration Bozdag, Mehmet; Urek, Ferhat; Cesur, Sumeyye; Sahin, Ali; Gunduz, Oguzhan The natural bone structure consists of three different nanocomposite layers; a porous polymer ceramic part, a lamellar, and a fiber-matrix composition gives the bone its unique physical and biological properties. During bone tissue regeneration bioactivity, and osteoinductivity are especially important with other parameters such as porosity, degradation rate, and cell adhesion. In this study, hydroxyapatite (HA) and bovine serum albumin (BSA) protein-loaded, polyvinyl alcohol (PVA) and chitosan (CH) nanofibers were fabricated via the electrospinning method. The mean diameters of PVA/CH/HA/BSA-5, PVA/CH/HA/BSA-10, and PVA/CH/HA/BSA-15 nanofibers were measured as 325.39 +/- 77.512 nm, 332.45 +/- 82.251 nm, 447.03 +/- 101.382 nm respectively, required porosity and properties for bone tissue engineering were considered achieved. BSA release profiles of BSA-5, BSA-10, and BSA-15 nanofibers were similar in terms of burst release which continued until the 12th hour, 58 %, 78 %, and 73 % of the BSA were released, respectively. After 72 h 100 % of BSA were released from all nanofibers. Cell viability tests showed that PVA/CH/HA/BSA nanofibers exceeded the control group in terms of cell viability by 119.9 %. In future bone injury treatment, PVA/CH/HA/BSA nanofibers can assist the healing process of cracks and fractures, and decrease the recovery time of bone as an alternative bone healing nanofiber.

2025-01-01T00:00:00Z Bicer,Mesude https://hdl.handle.net/20.500.12573/2491 2025-04-14T09:10:27Z 2024-01-01T00:00:00Z

Revolutionizing dermatology: harnessing mesenchymal stem/stromal cells and exosomes in 3D platform for skin regeneration Bicer,Mesude Contemporary trends reveal an escalating interest in regenerative medicine-based interventions for addressing refractory skin defects. Conventional wound healing treatments, characterized by high costs and limited efficacy, necessitate a more efficient therapeutic paradigm to alleviate the economic and psychological burdens associated with chronic wounds. Mesenchymal stem/stromal cells (MSCs) constitute cell-based therapies, whereas cell-free approaches predominantly involve the utilization of MSC-derived extracellular vesicles or exosomes, both purportedly safe and effective. Exploiting the impact of MSCs by paracrine signaling, exosomes have emerged as a novel avenue capable of positively impacting wound healing and skin regeneration. MSC-exosomes confer several advantages, including the facilitation of angiogenesis, augmentation of cell proliferation, elevation of collagen production, and enhancement of tissue regenerative capacity. Despite these merits, challenges persist in clinical applications due to issues such as poor targeting and facile removal of MSC-derived exosomes from skin wounds. Addressing these concerns, a three-dimensional (3D) platform has been implemented to emend exosomes, allowing for elevated levels, and constructing more stable granules possessing distinct therapeutic capabilities. Incorporating biomaterials to encapsulate MSC-exosomes emerges as a favorable approach, concentrating doses, achieving intended therapeutic effectiveness, and ensuring continual release. While the therapeutic potential of MSC-exosomes in skin repair is broadly recognized, their application with 3D biomaterial scenarios remains underexplored. This review synthesizes the therapeutic purposes of MSCs and exosomes in 3D for the skin restoration, underscoring their promising role in diverse dermatological conditions. Further research may establish MSCs and their exosomes in 3D as a viable therapeutic option for various skin conditions.

2024-01-01T00:00:00Z Bicer, Mesude https://hdl.handle.net/20.500.12573/2487 2025-04-11T14:50:53Z 2024-01-01T00:00:00Z

Revolutionizing dermatology: harnessing mesenchymal stem/stromal cells and exosomes in 3D platform for skin regeneration Bicer, Mesude Contemporary trends reveal an escalating interest in regenerative medicine-based interventions for addressing refractory skin defects. Conventional wound healing treatments, characterized by high costs and limited efficacy, necessitate a more efficient therapeutic paradigm to alleviate the economic and psychological burdens associated with chronic wounds. Mesenchymal stem/stromal cells (MSCs) constitute cell-based therapies, whereas cell-free approaches predominantly involve the utilization of MSC-derived extracellular vesicles or exosomes, both purportedly safe and effective. Exploiting the impact of MSCs by paracrine signaling, exosomes have emerged as a novel avenue capable of positively impacting wound healing and skin regeneration. MSC-exosomes confer several advantages, including the facilitation of angiogenesis, augmentation of cell proliferation, elevation of collagen production, and enhancement of tissue regenerative capacity. Despite these merits, challenges persist in clinical applications due to issues such as poor targeting and facile removal of MSC-derived exosomes from skin wounds. Addressing these concerns, a three-dimensional (3D) platform has been implemented to emend exosomes, allowing for elevated levels, and constructing more stable granules possessing distinct therapeutic capabilities. Incorporating biomaterials to encapsulate MSC-exosomes emerges as a favorable approach, concentrating doses, achieving intended therapeutic effectiveness, and ensuring continual release. While the therapeutic potential of MSC-exosomes in skin repair is broadly recognized, their application with 3D biomaterial scenarios remains underexplored. This review synthesizes the therapeutic purposes of MSCs and exosomes in 3D for the skin restoration, underscoring their promising role in diverse dermatological conditions. Further research may establish MSCs and their exosomes in 3D as a viable therapeutic option for various skin conditions.

2024-01-01T00:00:00Z